Chapter 1: Hardware Architecture
1.1 XY Linear Translation Stages
The Nanomaterials Laboratory uses Micos SMC Basic linear translation stages, which consist of X and Y axis pairs. These stages provide a translation step resolution of 0.5 µm and a maximum velocity of 1000 µm/s. They are positioned below the objective lens of an optical microscope (where the optical path is conventionally considered the Z-axis), enabling precise micrometer movement of the camera relative to the samples.
The stages are controlled by a microcontroller box that accepts a fixed set of serial commands from a user’s computer via an RS232 cable. Therefore, laptop users need an RS232 to USB adapter and relevant drivers (for Windows systems) to connect with the stages.
To simplify stage integration in programming, we developed a Python script that provides an interface for easier stage control. More details are provided in Section 2.
1.2 Optical Shutter
In our experiments, we often use a laser beam to cut a rectangular raster on our samples, focused through a modified microscope. With the XY linear translation stages moving the sample relative to the stationary laser beam, we can cut any pattern in the XY plane. To prevent the laser beam from entering the microscope between cuts, it was initially blocked manually with pulleys at the user's work area.
To automate this cutting process, we developed a shutter using a small 5V servo motor (SG90 9g). Typically, an integrated control (IC) board (such as Arduino) and a motor driver are required to power and control servo motors. To avoid purchasing costly circuit boards for a single motor setup, we devised an alternative approach:
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Servo Motor Inputs: Our servo motors have three inputs: positive voltage, negative voltage, and a signal terminal.
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Signal Acceptance: The signal is a DC Pulse Width Modulated (PWM) wave, where the duty cycle determines the motor's absolute position. Some servos are modified for full 360° rotation (SG90 9g 360), where the duty cycle controls speed and signal duration determines rotation time (i.e., non-absolute positioning).
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Computer Output: Computers output waveforms via audio channels, but most can only achieve a maximum of 2V through the audio port, whereas our servo requires 5V.
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Signal Amplification: We channel the computer's audio signal through an operational amplifier (OpAmp) circuit to boost the signal to 4V, enough to trigger the servo’s signal circuitry.
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Power Supply Setup: By adding a micro-USB port, we provide a 5V DC power supply for both the motor and OpAmp circuit. This allows servo control using a specialized circuit with a micro-USB power source and a 3.5mm stereo audio jack input, where the PWM signal is generated in situ when executing stage control scripts.
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System Naming: This setup is referred to as the Opto-Audiomechano (OA) shutter.
1.3 Neutral Density (ND) Filter Wheel
In our laboratory, ND filters are essential for attenuating laser light to achieve specific power levels. We currently use Thorlabs’s AR Coated Mounted Round Variable ND Filters (NDC-50C-2M-A). These filters are coated to provide optical densities (OD) directly proportional to the rotation angle, orthogonal to the incident light path.
However, the rotation is manually operated, requiring the user to adjust the wheel by hand while checking the laser power with a meter. By machining a custom acrylic axle for the wheel, we can integrate our ND filters with a 360° OA servo motor. This allows users to perform precise and programmable rotations, thus enabling automated power adjustments.